rabbit anti cox iv 3e11 antibody  (Cell Signaling Technology Inc)

Journal: Cell Reports Medicine

Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma

doi: 10.1016/j.xcrm.2024.101706

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Article Snippet: Rabbit anti-COX IV (3E11) monoclonal antibody , Cell Signaling Technology , Cat #: 4850; RRID: AB_2085424.

Techniques: Plasmid Preparation, Virus, Microarray, Recombinant, Modification, Isolation, Clinical Proteomics, Acid Assay, Amplex Red Cholesterol Assay, Activity Assay, Bicinchoninic Acid Protein Assay, Lysis, Protease Inhibitor, Luciferase, Transfection, cDNA Synthesis, Control, Software

Journal: Genome Biology and Evolution

Article Title: Expression of Truncated Products at the 5′-Terminal Region of RIPK2 and Evolutive Aspects that Support Their Biological Importance

doi: 10.1093/gbe/evae106

Figure Lengend Snippet: Human RIPK2 mRNA expression in cells under temperature stress conditions. a-b) RT-PCR assays were performed in triplicates to evaluate the level of human RIPK2 transcripts 1 and 2 in cells under heat/cold conditions. Temperature stress induced a significant increase in transcript 2 expression level at lower temperatures and a decrease at a higher temperature ( P < 0.0001, unpaired t test), but no effect on transcript 1 expression was observed (control cells at 37 °C as the calibrator sample; TUBA1C as the expression reference). c ) HSPA1A showed elevated levels at 40 °C ( P = 0.03, unpaired t test) ( TUBA1C as the expression reference. Values were log2 transformed (y-axis) so that all values below −1 indicate downregulation in gene expression while values above 1 represent upregulation. The error bar represents the mean ± S.E.M (standard error of the mean). d-f) Western blot analysis of human RIP-2 expression in cells under stress conditions by anti-RIP-2 ab8428 (N-terminus), cstD10B11 and ab57954 (C-terminus) antibodies. In FaDu cell line under temperature stress, a reduced isoform 1 expression was observed at 5 °C or 17 °C compared to control cells. Normalization controls: β-tubulin and COX IV. L = Protein Ladder.

Article Snippet: The antibodies against housekeeping proteins were (a) a rabbit monoclonal anti-COX IV (3E11) antibody (#4850, CST, diluted 1:1000); (b) a rabbit monoclonal anti-β-tubulin (9F3) antibody (#2128, CST, diluted 1:1000); (c) a rabbit monoclonal anti-histone H3 (D1H2) XP® antibody (#4499, CST, diluted 1:1000); (d) a rabbit monoclonal anti-GAPDH (D16H11) XP® antibody (#5174, CST, diluted 1:1000); (e) a mouse monoclonal anti-β-actin antibody (A5441, Sigma−Aldrich, diluted 1:5000).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Transformation Assay, Gene Expression, Western Blot